Preparation of a fat-like substance from the bodies of bacteria.



V. g e. DBYGKB. I I PBEPAEATZON OF A FAT LIKE SUBSTANCE FROM THE BODIES 0F BACTERIA.

APPLICATION TILED 08113, 1905,

BPBOIHEHB.

Patented Oct. 11, 1910.

UNITED STATES PATENT OFFICE.

GEORG DEYCKE, .OF CONSTANTINOPLE, TURKEY, ASSIGNOR TO THE FIRM OF KALLE AND COMPANY AKTIENGESELLSCHAFT, OF BIEBBICH, GERMANY.

PREPARATION OF A. FAT-LIKE SUBSTANCE FROM THE IBQDIES OF BACTERIA.

' Applicationflled October 8. 1908.

, To all whom it mag concern:

Be it known that I, GEORG Di ircirs, subject of the Emperor of Germany, and residing at. Constantinople, Turkey, have invented certain new and useful Improve merits in the Preparation of a Fat-Like Substance. from the Bodies of Bacteria, of which the following is a specification.

methods. by which my invention may be carried into effect.

Homogeneous cultures of Streptothrz'a: leproz'aes are washed with water, ground up with ammonia liquor, treated witlrAdams distillation.

solution (a mixture consisting of 110 parts by volume of ether, and 100 parts by volume of a mixture composed of 30 grams of amnioniac water specific gravity 0.9283-t grams of 90 per cent. alc0h01,-andv 136 grams of water,)' washed again and dried in a vacuum. The dry bacilli are very thoroughly extracted bv means of ether or other liquid utpplicable for the extraction of fats (chloro orni, ligroin, benzene, carbondisultid) the solid residue being repeatedly ground to obtain a complete disintegration of the hacillie mass. The extract in-solution being filtered off is then freed from ether by The residue of the distillation is dissolved in hot, alcohol. The hot alcoholic solution is filtered. On cooling -a whitish substance separates from the liquid. It is filtered oil and washed with cold alcohol and may be further purified, if necessary. by redissolving it in hot alcohol, separating it again by cooling, filtering and washing. In this manner I obtain a white solid substance, which on saponificat-ion proves ti.- be a gylcerin ester of a fatty acid. The new substance-is very easily soluble in ether and chloroform, dilticultly soluble in cold alcohol or wood spirits. It liquefies on heating. The mcltingpoiut of this sc -stance is between 48 and 52 (1. and it is not. easily aiicctcd by exposure to atmospheric air, a relatively long exposure being required to decompose it. fn this particular Specification of Letters I'atent.

Serial No. 331,255. (Specimen) Patented Oct. 11, 1910.

itisclearly distinguishable from other fats which on exposure to air quickly decompose and become rancid. Another distinguish.

ing characteristic of the substance is the form. in which it crystallizes out of olive oil, the crystals being white and like flat needles in form, as shown in Figure 1 of the IICCOIH' panying drawing. :Fig. 2 oft-he drawing illustrates the form in which the substance crystallizes out of alcohol.

In the drawing the crystals are shown as magnified about 25 times their natural size which is about i; millimeter.

The substance obtained as herein before described further possesses the following characteristics. It contains about 92% 0 fatty acids insoluble in. water (Hehners. figure), its saponification numeral lies at' 21 0 (1 mgr. KOH per 1 g. fat), takes up 142O volatile fatty acids and requires from zero' g. iodin per 100 g. fat, contains no to a maximum of 1.7 mg) per 1 g. KOH for eacsi grain of fat to aci the system of patients sufi'ering of lepra. or tuberculosis by subcutaneous injection, it shows a therapeutic effect on these diseases. It also produces inimunit against virulent tubercle-bacilli. The app ication of the new substance may be effected as follows: The substance is dissolved in olive oil (0.2 g. to 2.0 g. in 1000 cam. of olive oil). )ther suitable medicaments for exam le benc lchlorid, ins y be added to this so ution w ich is injected into the diseased organism, the

amount depending of course on the state of the disease or the extent to which it is developed. The effect of the substance employed in the manner described, may be re ferred to as acting to dissolve, in a bacteriological sense, the bodies of acid-proof bacteria. The danger for an organism infected by acid-proof bacilli, consists in the fact that neither the naturalprotective wers of the or anism nor the antitox'ical odies (bacterio ysins) formed in the organism by reason of the infection, are able to attack the bacteria themselves and destroythem, because. the baeil ii are protected against such attacks b their coating composed partially of fat an waxdike sabstances. If, iowever,

the substance herein described has been introduced into the organism, such coating of the bacilli is loosened or to such sorb the free fatty If the new substance is introduced into.

an extent that a complete bacteriolysis of the bacteria may take place. "In-this sense,

the substance is said to cause a dissolving of the bacteria In the exam-pie given above, other microhes 'for instance llllXIClQ-lHtCllll or other;

' bacteria yielding acid proof colorations with coal tar colors may be employed instead of washing the solids, drying them in vacuum,

subjecting the dried bacilli to the action of a fat. solvent, filtering the extract, distilling the extracting medium, and crystallizing the 1 residue from alcohol.

4. rue herein described processof pro 7 ducing from Strcptothria: Zeproides a fatlike substance having the properties-described, consistin r in washing thebacteria with water, grin ing them in ammonia liquor, treating with Adams solution, again washing the solids, drying them in vacuum,

subjecting the dried bacilli to the action of a fat solvent, filtering the'extract, distillingthe extracting medium, and crystallizing the residue from alcohol. V 3. The herein described fat-like substance obtained as described andlieing a solvent for virulent lepra and tuberculosis bacteria,

said substance crystallizing out of olive oil in the form .of white fiat needles, having its melting point at. 48 to 52 (3., being easily, soluble in ether and chloroform,almost in soluble in cold alcohol, soluble too highdegree in boiling alcohol, contains about 92% of fatty acids insoluble in Water, the SilFUnlfiCzlilOll numeral of winch lies at 210,

ta (es up 1420 g. iodin per 100 g. fat, con-.

tains no volatile fatty acids and requires, to absorb the free fatty acids, from zero to a maximum of 1.7 mgr. per 1 g. KOH for each grain of fat, exposure to atmospheric air.

GEORG DEYCKE.

. and not easily affected by Witnesses IGNAT Rosexmrno, Go'rrnou) Fisomzn. 

